| Originally published in:
Gartz, Jochen, Allen, John W., and Mark D. Merlin. 1994. Ethnomycology,
Biochemistry and Cultivation of Psilocybe samuiensis, A New Psychoactive
Fungi from Thailand. Journal of Ethnopharmacology. vol. 43(1):73-80.
|Several specimens of Psilocybe and Copelandia species
in Koh Samui, Thailand were recently collected by JWA and native residents
for herbarium deposit and scientific study. The following paper presents
an ethnomycological and biochemical study of one of the species; Psilocybe
samuiensis Guzmán, Bandala and Allen, a new psychoactive gill fungus reported
Mycelium for the cultivation of Psilocybe samuiensis was obtained on 6% malt agar from the spores of a dried specimen. The growth of Psilocybe samuiensis was similar to that of Psilocybe tampanensis Guzmán and Pollock; but grew more rapidly than the mycelium of Psilocybe semilanceata (Fr.:Sacc.) Kumm. Laboratory analyses indicates that the alkaloid content in cultured fruit bodies of Psilocybe samuiensis is in the same order of magnitude as that found in naturally occurring mushrooms of this species.
HPLC analyses of both naturally occurring and in vitro cultivated fruit bodies of Psilocybe samuiensis revealed high concentrations of psilocybin and psilocin. Small amounts of baeocystin were also detected. Psilocybin levels varied from 0.23% up to 0.90%. The psilocybin content was highest in the caps.
Psilocybin was also found in the cultured non-bluing mycelia of Psilocybe samuiensis and varied from 0.24% to 0.32% dry weight. The relative alkaloidal content of psilocybin, psilocin, and baeocystin found in Psilocybe samuiensis was similar to that measured in other psychoactive fungi, but completely different from that found in Psilocybe semilanceata.
|KEYWORDS: Psilocybe samuiensis, psilocybin, psilocin, cultivation, Koh Samui, Psilocybe sp., psychoactive fungi.|
|Recent ethnomycological investigations on Koh Samui
Island in Thailand (Allen, 1991; Allen and Merlin, 1992a, 1992b; Guzmán
et al., 1993), confirm reports that some species of psychoactive fungi
are ingested for recreational purposes by foreign tourists and some indigenous
people (see Allen et al., 1992).
Koh Samui (280 km2 located at 10° N latitude, 100° E longitude), is a small tropical Island in the Gulf of Siam where psychoactive fungi are harvested by local farmers and their children. The psychoactive dung fungi (Psilocybe cubensis (Earle) Singer and/or Psilocybe subcubensis Guzmán) are known locally as hed keequai (literally, "mushrooms which appear after water buffalo defecates"). These mushrooms are sold by some farmers or their families directly to tourists and resort restaurants. At some resort restaurants the fungi are offered in a variety of meals (Allen and Merlin, 1992a).
Fungi specimens collected on Koh Samui Island (1991) for herbarium deposit and scientific study include the following species: Psilocybe cubensis, Psilocybe subcubensis, Copelandia cyanescens (Berkeley et Broome) Singer, and a previously unreported bluing Psilocybe species, Psilocybe samuiensis Guzmán, Bandala and Allen, sp. nov. (fig. 1). Chemical investigations of the psychoactive properties of some of these fungi include an unpublished study by Stijve (Nestec laboratories, Vevey, Switzerland) who analyzed Koh Samui collections of Psilocybe cubensis and Psilocybe samuiensis, as well as a Swiss collection of Psilocybe semilanceata (Fr.:Sacc.) Kumm. Tryptamine indoles were found in all three species. A second study by the authors was later conducted utilizing naturally occurring material and material grown only in vitro. Results of the HPLC and TLC analysis of both studies of Psilocybe samuiensis revealed high concentrations of tryptamine indole alkaloids. Furthermore, the senior author (JG) was successful in isolating a pure strain of Psilocybe samuiensis on malt agar and grass seed.
|CAP:7-15 mm in diameter,
subconvex to conic-convex, conic umbonate or campanulate-umbonate, frequently
with a small papilla. Viscid with a separable pellicle, even and striate
to sulcate at the margin. Hygrophanous. Chestnut or reddish-brown to straw-color,
becoming pale straw-color or brownish clay when dry.
GILLS:Adnate to adnexed, clay color, becoming violaceous brown or chocolate brown-violet when dry, with white edges.
STIPE:40-65 x 1.52 mm, equal or slightly subbulbous. Hollow. White or whitish to pale straw color. Covered with white fibrils. Context concolorous with pileus, bluing with slightly farinaceous taste and odor.
HABITAT: Psilocybe samuiensis was first collected in soil of mixed sand and clay, among fan palms in rice paddies situated 2 km west of the village of Ban Hua Thanon, Koh Samui, Thailand. Unlike Copelandia, and some species of Psilocybe which are coprophilous, Psilocybe samuiensis does not fruit directly in manure but appears scattered or gregarious in the manured soil of rice paddies. This fungus fruits from early July through late August.
While foraging for psychotropic fungi on Koh Samui
island in August of 1991, John W. Allen, accompanied by several Samui
children, harvested Psilocybe samuiensis for herbarium deposit and scientific
examination (See Guzmán et al., 1993). Psilocybe samuiensis first attracted
the attention of the collector (JWA) because of its macroscopic similarity
to Psilocybe semilanceata (the "liberty cap" mushroom). Both can be
found in similar environments (i. e., pasture lands, rice paddies, etc.),
occurring in the manured soil of ruminants. Psilocybe samuiensis was
then photographed in situ, as were several carpophores of Psilocybe cubensis
and/or Psilocybe subcubensis (which is macroscopically indistinguishable from
Psilocybe subcubensis), and a few specimens of Copelandia cyanescens which were also
observed growing in the same rice paddy where Psilocybe samuiensis occurred.
|Mycelium was obtained from the spores of a dried
specimen of Psilocybe samuiensis by methods described by Stamets and Chilton
(1983) and was stored as stock culture on 6% malt agar. Strains on agar
of a related species Psilocybe tampanensis Guzmán and Pollock and Psilocybe semilanceata
from Germany were also obtained. In a ratio of 1 to 6% on malt agar, the
whitish mycelium of Psilocybe samuiensis grew at a faster pace than that of similar
mycelium of Psilocybe semilanceata. The rapid growth of Psilocybe tampanensis was similar
to the growth of Psilocybe samuiensis; however, the former species soon formed
brownish sclerotia on the agar as it does with Psilocybe mexicana (Stamets and
Chilton 1983). Even after a relatively long growth period (3 months),
the mycelium of Psilocybe samuiensis formed only a few small brownish sclerotia.
Similar conditions were observed while cultivating the three species on Lolium seed / water (1:1.5). The same conditions were also observed in complete darkness; and Psilocybe tampanensis and Psilocybe samuiensis both grew with rapid speed. Observations on the rapid formations of sclerotia in Psilocybe tampanensis after a few weeks of cultivation has already been reported by Stamets and Chilton (1983). In contrast, Psilocybe samuiensis under cultivation only formed thick whitish mycelium (rhizomorphs, diameter 2 to 3 millimeters) throughout the media, and produced no sclerotia. Under the same conditions of cultivation, Psilocybe semilanceata grew slowly, producing only a fine and whitish mycelium with no formation of sclerotia or rhizomorphs.
Psilocybin was found to be present in the cultured, non-bluing mycelium of Psilocybe samuiensis grown on 6% malt agar. Amounts of psilocybin, ranging from 0.24 to 0.32% dry weight, were analyzed in 5 different batches of mycelium grown over a four week period. Analyses also revealed that these quantities of psilocybin were much lower than those detected in the naturally occurring fruit bodies obtained from the field. Interestingly, no other indole derivatives were detected in the extracts of the in vitro grown mycelium.
The alkaloidal levels obtained from the slightly bluing sclerotia of Psilocybe tampanensis were high. Additionally, the amount of psilocybin obtained from five different cultivations grown on 6% malt agar and Lolium seed ranged from 0.34 to 0.68% by dry weight, and from 0.41 to 0.61% in three sections of sclerotia obtained from a single cultivation on Lolium seed. The sclerotia obtained from malt agar also contained 0.21 to 0.52% psilocin, but no baeocystin was detected. The sclerotia obtained from Lolium had a concentration of psilocin from 0.11 to 0.32%. Until now, it was not possible to produce complete fruit bodies of Psilocybe samuiensis on either malt agar or Lolium seed. Some small incomplete fruit bodies of Psilocybe samuiensis (up to 2 centimeters high) appeared, but failed to develop into normal sporulating mushrooms. These premature formations only occurred on agar with a low concentration of malt (0.5 to 1.5%). After stopping their natural growth, these incomplete fruit bodies began to exhibit a slight spontaneous bluing reaction.
< br> At this time it was not possible to be able to cultivate mushrooms on a Lolium seed / water mixture (Stamets and Chilton. 1983). Psilocybe samuiensis also grows well on some grains such as rye or rice. A mixture of rye/horse dung/water (2:1:2) did produce fruit bodies of Psilocybe samuiensis after 4 months cultivation, and 3 weeks after casing with peat/chalk (2:1) (Stamets and Chilton, 1983) (fig. 2). Two flushings producing eight mushrooms were observed; six of the mushrooms were analyzed (see fig. 2 and table 1).
|Two separate chemical studies were undertaken to
determine the tryptamine alkaloid content of Psilocybe samuiensis. The
first involved naturally occurring field specimens; the second analyzed
material cultivated in the laboratory.
In the first study, 15 specimens of naturally occurring fruit bodies of Psilocybe samuiensis were analyzed by HPLC and TLC techniques (Gartz, 1987). High amounts of psilocybin were detected (0.23% - 0.90% dry weight); and a few specimens contained similar amounts of psilocin (0.05% - 0.81% dry weight). Baeocystin, a precursor to psilocybin, was also detected (0.01% - 0.5% dry weight) in all naturally occurring specimens of Psilocybe samuiensis but in much smaller concentrations than psilocybin. This is in sharp contrast to the high concentrations of baeocystin and very small amounts of psilocin (only in a few specimens) which were detected in naturally occurring field specimens of Psilocybe semilanceata from various origin (Gartz, 1993), and in vitro cultivated fruit bodies of Psilocybe semilanceata (Gartz, 1991a, 1991b).
In contrast to the cultivated Psilocybe cubensis (Gartz, 1987), where the accumulation of psilocin is often higher in the stems than in the caps, analyses of Psilocybe samuiensis revealed that the caps contained more psilocybin than the stems. Identical concentrations of the alkaloids (psilocybin, psilocin, and baeocystin) were found in the cultivated fruit bodies of Psilocybe samuiensis and Psilocybe semilanceata grown in rye/horse dung (Gartz, 1991a, 1991b). Stijve also found similar concentrations of psilocin and psilocybin in 5 naturally occurring fruit bodies of Psilocybe samuiensis (collection F, 8 August, 1991, psilocybin, 0.14%; psilocin, 50%; baeocystin, <0.01%).
Plate I (fig. 3) reveals the qualitative results of analysis of Psilocybe samuiensis (collection F, 8/8/91), along with the comparative study of 2 Thai collections of Psilocybe cubensis (collection C and G, August 3, 1991 and August 8, 1991), and analysis of a Swiss collection of Psilocybe semilanceata. Analyses were performed by Thin-Layer Chromatography (TLC) on cellulose 10 x 20 cm Nano plates. BAW=Butanol acetic acid-water 60:15:25 V/V. pDMCA reagent.
Plate II (fig. 4) reveals the qualitative results of analysis of Psilocybe samuiensis (collection F, August 8, 1991), a Swiss collection of Psilocybe semilanceata and a Thai collection of Psilocybe cubensis (collection G, August 8, 1991). Analyses were performed by Thin Layer Chromatography (TLC) on NANO-cellulose 10 x 10 cm., N-propanol-10% ammonia 5:2 V/V., PDMCA-reagent.
|Previous studies by Allen and Merlin (1992a, 1992b)
and Guzmán et al. (1993) confirm reports that several species of psychoactive
fungi in Thailand are used for non-traditional recreational purposes.
The most commonly used species is Psilocybe cubensis (and/or Psilocybe subcubensis).
Although psychoactive fungi are currently illegal in Thailand, such use
is still common at many resorts on Koh Samui, Koh Pha-Ngan, and possibly
other areas of this country. Allen and Merlin (1992) also reported that
some adults as well as some children have eaten (or attempted to smoke)
psychoactive fungi species for recreation. Furthermore, some tourists
have apparently influenced a small segment of native inhabitants, who
are enticed by their foreign companions into consuming such fungi. Foreign
visitors may well have been responsible for introducing the use of psychoactive
fungi to Koh Samui and other resort areas in South and Southeast Asia.
During the collection of field specimens, JWA questioned native children and a few adults whom he encountered in the rice paddies concerning their relationship with "hed keequai" and other fungi found in their environment. Some children were aware of numerous varieties of edible fungi as well several poisonous and psychoactive fungi species occurring on Koh Samui. On one occasion, several children warned JWA not to eat Panaeolus antillarum (Fr.) Dennis, explaining that it was "antaray" (dangerous). A toxin is not known from this species at present. The authors were unable to confirm if the collection of psychoactive fungi by Samui farmers and their families had caused any serious poisonings due to the possible misidentification of species. However, the native farmers and their children are very knowledgeable regarding the natural flora of their environment. Regarding the new species, Psilocybe samuiensis is readily differentiated from other mind-altering mushrooms by a few adults and some native children who frequently collect psychoactive dung fungi. However, it is not known if this species is harvested for human consumption by foreign tourists or immigrants living on Koh Samui Island. It is possible that some European mycophagists may have collected and experimented with this species after noticing its macroscopic similarity to P. semilanceata. A few children and one adult apparently recognized fresh carpophores of Psilocybe samuiensis (harvested by JWA) as a unique type of fungus. At least some of the Samui cattle tenders and their children are aware that the psychoactive effects of Psilocybe samuiensis are similar to the mind-altering effects of the larger specimens of "hed keequai" (i.e., Psilocybe cubensis), which they often gathered. However, when compared to Psilocybe cubensis, Psilocybe samuiensis is a small inconspicuous fungus, not more than 2-3 inches in height and can be easily overlooked by both tourist and native collectors seeking the larger specimens of Psilocybe cubensis.
After noticing that several carpophores of Psilocybe samuiensis exhibited a slight bluing reaction after handling, JWA bioassayed 25 fresh specimens (weighing approximately 6 grams wet weight); This resulted in an intensely visual experience similar to the action of equal amounts of Psilocybe semilanceata from Germany.
Prior to the botanical identification of Psilocybe samuiensis by Guzmán et al. (1993), a small collection of Psilocybe samuiensis was sent to Dr. T. Stijve of Nestec Ltd., Vevey, Switzerland for botanical identification. Unable to properly identify the fungi, Stijve (Pers. Comm., 1992a) forwarded several carpophores of Psilocybe samuiensis to Klaus Hřiland of the Botanical Garden and Museum in Oslo, Norway. Hřiland, in a personal communication to Stijve (11 June 1992), reported that he "examined the dried specimens according to Guzmán's taxonomic key of the genus Psilocybe," suggesting that the dried material "corresponded to Psilocybe mexicana Heim or a very closely related species." Furthermore, Hřiland suggested that "since it [Psilocybe mexicana] is only known [of] from [the North] America[n] [continent], care should be undertaken to accept the species from Thailand. It may occur there naturally [Koh Samui], or it may have been introduced by people from [North] American samples, or it is a close, but undescribed species (Stijve, Pers. Comm. to JWA, 12 June 1992)."
Psilocybe samuiensis is microscopically similar to Psilocybe mexicana, but the form and size of the spores, as well as the presence of pleurocystidia, its macroscopic features and the habitat, are very close and somewhat similar to Psilocybe semilanceata. Guzmán et al. (1993) placed this species in the section Mexicanae because of the big rhomboid or subrhomboid spores which separate this species from P. mexicana and other species in the Psilocybe section Mexicanae; it is the first species of that section to be found outside of the New World.
The chemical composition of Psilocybe samuiensis is also quite different than that of P. semilanceata which contains much more baeocystin than P. samuiensis (Gartz, 1991a, 1991b, 1993).
Both species are macroscopically distinguished and/or separated by the height or length of their respective stipes and the color of the fruit bodies. Psilocybe samuiensis attains a height of from 40-65 x 1.52 mm and Psilocybe semilanceata has a natural height of from 70-110 x 1.52 mm. During crossing experiments, complete reproductive barriers have been found between 4 mono karyons of Psilocybe semilanceata from Germany (2) and Austria (2), and in (3 strains of) Psilocybe samuiensis from Thailand. It is clear that both are autonomous species which do not form hybrid dikaryons.
Recent chemical analyses of both naturally occurring and cultivated specimens of Psilocybe samuiensis by the authors, as well as analysis of 5 naturally occurring fruit bodies by Stijve, indicate that it is a relatively potent psychoactive species containing high concentrations of both psilocybin and psilocin.
|Duplicate collections of fungi specimens referred to in this study (collected August 2-11, 1991) have been deposited at the Instituto de Ecologia in Xalapa, Veracruz, México (including Psilocybe samuiensis, holotype XAL, Allen F, 1991) and at the Pacificum Herbarium in the Bernice P. Bishop Museum in Honolulu, Hawaii (including Psilocybe samuiensis, isotypes in BISH and 0, Allen F, 626452, Allen F1, 626825). Additional specimens were sent to Dr. Rolf Singer, Field Museum of Natural History, Chicago, Illinois, and to Dr. Prakitsin Sihanonth, Chulalongkorn University, Bangkok, Thailand.|
|The authors wish to express their gratitude to Dr. Tjakko Stijve of Nestec Ltd, Vevey, Switzerland for his chemical analysis of the Thailand fungi. Appreciation is also extended to Dr. Klaus Hřiland for first examining specimens of Psilocybe samuiensis, and to Dr. Gastón Guzmán of the Instituto de Ecologia, Xalapa, México and Dr. Ewald Gerhardt of the Botanisches Museum, Berlin, Dahlem, Germany for their identification of the Thailand material. In addition, the authors wish to express their gratitude to Jonathan Ott, Natural Products, Xalapa, México, for his time and consideration in kindly reviewing this manuscript.|
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Gartz, J. (1991a) Further investigations on psychoactive mushrooms of the genera Psilocybe, Gymnopilus, and Conocybe. Annali dei Musei Civici Rovereto vol. 7:265-274. (Italy).
Gartz, J. (1991b) Einflusse von Phosphat auf Fruktifikation und Sekundärmechanismen der Myzelien von Psilocybe cubensis, Psilocybe semilanceata und Gymnopilus purpuratus. Z Mykol vol. 57:149-154.
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Psilocybin Psilocin Baeocystin
1. 0.58---- 0.34---- 0.02
2. 0.43---- 0.21---- 0.03
3. 0.36---- 0.52---- 0.04
4. 0.47---- 0.31---- 0.04
5. 0.62---- 0.23---- 0.05
6. 0.73---- 0.25---- 0.03
|Figure 1. Fresh harvested specimens of Psilocybe
samuiensis Guzmán, Bandala and Allen. Ban Hua Thanon, Koh Samui. Photograph
by John W. Allen. Photograph is actual size.
Figure 2. Psilocybe samuiensis Guzmán, Bandala and Allen. Grown on rye/horse dung. Photograph by Jochen Gartz.
Figure 3. Plate I. TLC analytical results are 1=psilocin. 2=turquoise spot often encountered in extracts of hallucinogenic mushrooms. 3=psilocybin. 4=baeocystin. Photograph by T. Stijve.
Figure 4. Plate II. TLC analytical results are 1=psilocin (to front). 2=metabolite characteristic of Psilocybe cubensis; resembles tryptophan, but does not match in other systems. 3=turquoise spot. 4=psilocybin. 5=baeocystin. Photograph by T. Stijve.